Abstract

The National Institute on Aging Interventions Testing Program (ITP) evaluates agents hypothesized to increase healthy lifespan in genetically heterogeneous mice. Each compound is tested in parallel at three sites, and all results are published. We report the effects of lifelong treatment of mice with four agents not previously tested: Protandim, fish oil, ursodeoxycholic acid (UDCA) and metformin - the latter with and without rapamycin, and two drugs previously examined: 17-α-estradiol and nordihydroguaiaretic acid (NDGA), at doses greater and less than used previously. 17-α-estradiol at a threefold higher dose robustly extended both median and maximal lifespan, but still only in males. The male-specific extension of median lifespan by NDGA was replicated at the original dose, and using doses threefold lower and higher. The effects of NDGA were dose dependent and male specific but without an effect on maximal lifespan. Protandim, a mixture of botanical extracts that activate Nrf2, extended median lifespan in males only. Metformin alone, at a dose of 0.1% in the diet, did not significantly extend lifespan. Metformin (0.1%) combined with rapamycin (14 ppm) robustly extended lifespan, suggestive of an added benefit, based on historical comparison with earlier studies of rapamycin given alone. The α-glucosidase inhibitor, acarbose, at a concentration previously tested (1000 ppm), significantly increased median longevity in males and 90th percentile lifespan in both sexes, even when treatment was started at 16 months. Neither fish oil nor UDCA extended lifespan. These results underscore the reproducibility of ITP longevity studies and illustrate the importance of identifying optimal doses in lifespan studies.

(http://www.autoadmit.com/thread.php?thread_id=3275901&forum_id=2],#30859950)

A �miracle drug� found in the soil at Easter Island is believed to have qualities that could one day help humans live longer � and it may already be working for dogs.

Scientists from the University of Washington are testing the effects of a drug called rapamycin on dogs to see if it will slow down the aging process.

Researchers were shocked by results of the initial trials, finding that some dogs showed improved heart functionality after just a few weeks.

Scientists from the University of Washington are testing the effects of a drug called rapamycin on dogs to see if it will slow down the aging process. Researchers were shocked by results of the initial trials, finding that some dogs showed improved heart functionality after just a few weeks.

Scientists from the University of Washington are testing the effects of a drug called rapamycin on dogs to see if it will slow down the aging process. Researchers were shocked by results of the initial trials, finding that some dogs showed improved heart functionality after just a few weeks

WHAT IS RAPAMYCIN?

Rapamycin is a bacterial by-product discovered in the shadows of the Easter Island's famous statues.

It is already used in transplant patients to prevent organ rejection and scientists say it can improve learning and help treat cognitive decline.

However, it comes with some serious side effects. For instance, the compound suppresses the immune system and makes patients vulnerable to any viruses and bacteria.

The existing version of the drug also increases the risk of cancer and would need to be modified before using in human trials.

The study is led by biologist Matt Kaeberlein and his colleague, Daniel Promislow.

According to Fusion, the researchers began clinical trials this year, expanding on earlier findings that rapamycin increases the lifespan of mice.

Rapamycin is a bacterial by-product discovered in the shadows of the island's famous statues and is named after Rapa Nui, the Polynesian name for Easter Island.

Dogs age very quickly compared to a human lifespan; most live between 10 and 13 years.

This allows researchers to study the entire aging process in a short amount of time.

The team recruited 40 dog-owners, who were each to give their pets three tablets of rapamycin a week, Fusion reports.

Rapamycin is a bacterial by-product discovered in the shadows of the island's famous statues and is named after Rapa Nui, the Polynesian name for Easter Island, pictured above. It is already used in transplant patients to prevent organ rejection and scientists say it can improve learning and help treat cognitive decline

Rapamycin is a bacterial by-product discovered in the shadows of the island's famous statues and is named after Rapa Nui, the Polynesian name for Easter Island, pictured above. It is already used in transplant patients to prevent organ rejection and scientists say it can improve learning and help treat cognitive decline

After the researchers weeded out dogs with heart conditions and other medical factors, they were left with 24 middle-aged dogs, who would each receive low doses of the drug.

This continued over the course of 10 weeks, and the researchers took echocardiograms throughout to determine any changes in the animal�s heart function.

The team discovered that dogs receiving rapamycin showed improved heart functionality, or showed no change.

And, those who had come in with worse conditions initially saw the most improvement, Fusion reports.

Kaeberlein says the results are 'astonishing' and he was 'shocked' when he got the data.

The team discovered that dogs receiving rapamycin showed improved heart functionality, or showed no change. And, those who had come in with worse conditions initially saw the most improvement. Moving forward, the researchers plan to conduct further tests with the drugs that will span multiple years.

The team discovered that dogs receiving rapamycin showed improved heart functionality, or showed no change. And, those who had come in with worse conditions initially saw the most improvement. Moving forward, the researchers plan to conduct further tests with the drugs that will span multiple years

Rapamycin is already used in transplant patients to prevent organ rejection and several years ago, and scientists in the journal Neuroscience said it can improve learning and help treat cognitive decline.

In mice, it has been shown to extend the lives of mice by 13 per cent in females and nine per cent in males.

Now, the researchers say it may help dogs live longer as well.

�It is almost certain that rapamycin can slow the effects of aging in dogs and people,� Kaeberlein told Fusion.

�The only question is whether it can slow aging with doses that don�t have significant side effects.�

Moving forward, the researchers plan to conduct further tests with the drugs that will span multiple years � and they hope it will be approved for humans in the next decade.

(http://www.autoadmit.com/thread.php?thread_id=3275901&forum_id=2],#30860313)

CR

(http://www.autoadmit.com/thread.php?thread_id=3275901&forum_id=2],#38991690)

Anti autism anti ocd anti rut

(http://www.autoadmit.com/thread.php?thread_id=3275901&forum_id=2],#38992206)

I wish I had been able to get a measurement of my DNA methylation/ epigenetic clock done before starting. I am surprised no one has funded a study to measure this in humans on metformin and/or rapamycin. It would be possible to quantify the health benefits just by looking at how gene expression evolves over a few months.

(http://www.autoadmit.com/thread.php?thread_id=3275901&forum_id=2],#34007335)

Rapamycin doesn't have nasty side effects at normal dosages. You could do transient dosing once a week if you don't want it in your system all the time

(http://www.autoadmit.com/thread.php?thread_id=3275901&forum_id=2],#38991672)

2) metformin doesn't really work either as it dulls the effect of exercise.

David Sinclair has clarified this.

(http://www.autoadmit.com/thread.php?thread_id=3275901&forum_id=2],#38872453)

What people need to realize is this is all research and theoretical and nobody needs to start drugging themselves for all this unproven stuff.

Just take care of yourself. In a few decades when actual anti-aging treatments are available that WORK, you will still be young enough to benefit if you're about 40 or under today.

(http://www.autoadmit.com/thread.php?thread_id=3275901&forum_id=2],#38872465)

There is a high probability rapamycin can delay aging in humans. It consistently works in a wide range of species, the mtor pathway is conserved in humans and genes related to it are tied to longevity. There was a recent study showing slowed epigenetic aging in human cell cultures with rapamycin. The concern with rapamycin is the side effect profile, not its efficacy

(http://www.autoadmit.com/thread.php?thread_id=3275901&forum_id=2],#38990216)

David only takes Metformin on days he doesn't exercise because it dulls the benefit of exercise. Rapamycin is considered very risky.

(http://www.autoadmit.com/thread.php?thread_id=3275901&forum_id=2],#38991682)

There are legitimate concerns about rapamycin, but it seems reasonably safe at low doses. The glucose intolerance issues don't seem like a real problem at least

(http://www.autoadmit.com/thread.php?thread_id=3275901&forum_id=2],#38991799)

15% lifespan extension in marmosets, which makes human effects much more likely.

https://www.rapamycin.news/t/breaking-15-healthy-lifespan-improvement-via-rapamycin-seen-in-marmosets/14325

this is a study with pretty small dosing. 5-10mg over 48 weeks, but due to poor bioavailability the 10mg dosing was likely only 3mg a week, which is quite small. kind of a stupid study but they still managed to see improvements in lean mass in women. safety results are encouraging, with minimal side effects.

https://pmc.ncbi.nlm.nih.gov/articles/PMC12074816/

improved immune system function and reduce DNA damage in older adults (consistent with the prior vaccine research):

https://www.biorxiv.org/content/10.1101/2025.08.15.670559v1.abstract

improved cardiac function in older adults. not placebo controlled but the effects are consistent with mice and are likely clinically significant.

https://link.springer.com/article/10.1007/s11357-025-01855-8

signs of improved brain health in APOE4 carriers taking 7mg weekly.

https://pmc.ncbi.nlm.nih.gov/articles/PMC11957208/

i think it's reasonable for oldmos (of which there are now many on this board) to begin experimenting with it. risk of side effects from intermittent use are low. it's riskier to not be taking it given all of the evidence so far.

(http://www.autoadmit.com/thread.php?thread_id=3275901&forum_id=2],#49289892)

it gives you transient full blown AIDS basically

(http://www.autoadmit.com/thread.php?thread_id=3275901&forum_id=2],#49289934)

(http://www.autoadmit.com/thread.php?thread_id=3275901&forum_id=2],#49290139)